RePeat is a unique solid phase extraction cartridge (SPE) designed for repeated extractions of drugs from complex matrices, such as plasma, serum, milk, supernatants of cell cultures and fermentation broth. In contrast to ordinary disposable solid phase extraction columns, each RePeat cartridge can be used for a large number of samples. This will highly reduce the cost per sample.

RePeat is based on polymeric particles with a hydrophobic internal surface and a biocompatible external surface. The biocompatibility has been obtained by attachment of the plasma protein a1- acid glycoprotein (AGP) on the external surface of the particles. Immobilized AGP is an extremely stable protein, which tolerates all the organic solvents used in off-line solid phase extractions (SPE). The pores of the particles are small enough to exclude the plasma proteins and other macromolecular compounds, whereas drug molecules and other low-molecular-mass compunds can penetrate the pores and be adsorbed to the hydrophobic inner surface.

Since RePeat is polymer based, the cartridge can be used between pH 2-13. This property will give possibilities of extracting ionized analytes in their uncharged form. The uncharged analyte has higher affinity to the hydrophobic inner surface of the RePeat particle, giving an improved recovery.

RePeat is availiable in 1.5 ml (25 mg) cartridges.

• How to use RePeat?
• Applications
• Ordering information

Please contact ChromTech if you have any questions or if you need more information about the above applications.

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