Drug-plasma protein binding studies (drug-protein binding) using HPLC The degree of drug - plasma protein binding (drug-protein binding) gives significant effect on the pharmacokinetic and the pharmacodynamic properties of a drug. This is due to the fact that the unbound drug can penetrate the wall of the blood vessel, whereas the protein bound drug can not. Further more, the drug plasma protein binding is also stereoselective in nature due to the inherent chirality of plasma proteins like human serum albumin (HSA) and alpha-1-acid-glycoprotein (AGP). Many different methods are available for the determination of drug-plasma protein binding i.e. equilibrium dialysis and ultra filtration e.t.c. These methods suffer from many disadvantages, for example, they can not be automized, they are time consuming e.t.c. HPLC, however, is a convenient method to use for the determination of the degree of drug-plasma protein binding. ChromTech supply a range of HPLC columns that can be used for these kind of studies: columns based on human serum albumin (HSA), alpha-1-acid glycoprotein (AGP), rat serum albumin (RSA), dog serum albumin (DSA) and mouse serum albumin (MSA). More information on the use of these columns can be found under "How to use these columns" HSA - human serum albumin   DSA - dog serum albumin AGP - alpha-1-acid glycoprotein   MSA - mouse serum albumin RSA - rat serum albumin   Albumins from other species are also avaliable. Additional product lines: Chiral separation On-line extraction bioanalysis Solid phase off-line extraction

ChromTech Ltd, No. 2 The Courtyard, Greenfield Road, Congleton, Cheshire, CW12 4TR, U.K. Phone: +44 (0)1260 270 153, Fax: +44 (0)1260 274 394, Email: sales@chromtech.co.uk