CHIRAL-HSA

A chiral HPLC-column for direct resolution of enantiomers

The CHIRAL-HSA column is based on human serum albumin (HSA). The protein has been immobilized onto spherical 5 micrometer particles. Enantiomers of preferentially acidic compounds can be resolved directly, without derivatization. CHIRAL-HSA is especially suited for the resolution of weak and strong acids, zwitterionic and nonprotolytic compounds. However, basic compounds have also been resolved.

A reference list with literature references is presented.

In the Application Index application examples are presented.

Application Areas
At pharmaceutical companies, hospitals, universities and chemical industry CHIRAL-HSA is used for the analysis of enantiomeric purity and for bioanalysis.

Mobile Phases
The column is operated in the reversed phase mode. Phosphate buffers with addition of organic modifiers are used as mobile phases. Retention and enantioselectivity can be regulated by changing the mobile phase composition. The addition of octanoic acid is necessary when chromatographing for example arylpropionic acid derivates on CHIRAL-HSA.

Dimensions
CHIRAL-HSA columns are available with inner diameters of 2, 3, 4 and 10 mm in lengths of 50, 100 and 150 mm. Guards with the length of 10 mm and the inner diameters 2, 3 and 4 mm are available. Complete list of availible column dimensions

If you have any questions regarding the above, don´t hesitate to contact ChromTech!

Return to ChromTech´s Homepage