CHIRAL-CBH

A chiral HPLC-column for direct resolution of enantiomers

Cellobiohydrolase (CBH) is a very stable enzyme, which has been immobilized onto 5 micrometer spherical silica particles. This is a reversed-phase column, used for the direct separation of enantiomers. The column is preferantially used for the separation of enantiomers of basic drugs from many compound classes. CHIRAL-CBH separates preferentially compounds containing one or more basic nitrogen together with one or more hydrogen accepting or hydrogen donating groups (alcohol, phenol, carbonyl, amide, ether, sulphoxide, ester etc.).

A reference list with literature references is presented.

In the Application index application examples are presented. The chromatograms are demonstrated together with information about the column used and the mobile phase conditions.

Application Areas
At pharmaceutical companies, hospitals, universities and chemical industry CHIRAL-CBH is used for the analysis of enantiomeric purity and for bioanalysis. An interesting and fast growing area is isolation of pure enantiomers on semipreparative columns.

Mobile Phases
The mobile phases are mixtures of phosphate or acetate buffers and organic solvents as 2-propanol or acetonitrile containing 50mM disodium EDTA. The retention and the enantioselectivity can be regulated by changes in pH, buffer concentration and organic modifier (nature and concentration).

Dimensions
CHIRAL-CBH columns are available with inner diameters 2, 3, 4, and 10 mm in lengths of 50, 100 and 150 mm. Guards with length of 10 mm and the inner diameters 2, 3 and 4 mm are also available. Complete list of availible column dimensions

If you have any questions regarding the above, don´t hesitate to contact ChromTech!

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