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Extraction of the AstraZeneca compound (NAE-111) using BioTrap 500 C18 / MS-detection
The BioTrap 500 C18 online-extraction column
can be used with MS-detection, which is demonstrated in the
chromatogram below.
Courtesy Astra Arcus, Sweden
| Sample: |
NAE-111 (Astra compound) |
| Inj. vol.: |
50 µl serum |
| Extraction column
: |
BioTrap 500 C18, 20x4.0 mm |
| Extraction mobile
phase: |
2.5% 2-propanol in 10 mM sodium phosphate
buffer, pH 7.0 |
| Analytical column: |
Hypersil BDS C18, 100 x 4.6 mm, 3 µm |
| Analytical mobile phase: |
17% acetonitrile, 0.1% formic acid |
Analysis Program
| Switching valve position |
Analysis step |
Time |
| 1. Extraction position |
sample extraction |
3 min |
| 2. Elution position |
transfer of analyte |
3 min |
| 3. Extraction position |
separation and reequilibration |
3 min |
More information about BioTrap 500
Ordering information
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