CHIRAL-AGP (USP chiral column L41)
A chiral HPLC-column for direct resolution of enantiomers
The chiral selector in the CHIRAL-AGP phase is alpha1-acid
glycoprotein (AGP). This very stable protein has been immobilized
onto spherical 5 micrometer silica particles. The protein
tolerates high concentrations of organic solvents, high and low
pH, and high temperatures. The column is used in the
reversed-phase and can be used for direct resolution of
enantiomers, without derivatization. The CHIRAL-AGP column
separates enantiomers of an extremely broad range of drug
compounds:
- amines (primary, secondary, tertiary, and quaternary)
- acids (strong and weak)
- nonprotolytes (amides, esters, alcohols, sulphoxides,
etc.)
CHIRAL-AGP is most likely the column with the broadest
applicability of all chiral columns available today.
A reference list containing 202
literature references is presented.
In the Application index hundreds of
application examples are presented. The chromatograms are
demonstrated together with information about the column used and
the mobile phase conditions.
Application Areas
At pharmaceutical companies, hospitals, universities and chemical
industry CHIRAL-AGP is used for the analysis of enantiomeric
purity and for bioanalysis. A growing application area, due to
the exceptional applicability of the phase, is isolation of pure
enantiomers on semipreparative columns.
Mobile Phases
Very simple mobile phase systems are used for the majority of the
separations; buffered waterbased mobile phases with the addition
of low concentrations of an organic modifier, such as 2-propanol
or acetonitrile for example. The changes affect both the solute
and the protein on the stationary phase. The changes affect
different types of solutes in a different ways. Most of the
separations (>95%) have been performed using the above
described simple mobile phases. However, in some cases charged
modifiers as DMOA (N,N-dimethyloctylamine), octanic acid and
quaternary ammonium compounds are used to induce
enantioselectivity.
Dimensions
CHIRAL-AGP columns are available with inner diameters 2, 3, 4,
and 10 mm in lengths of 50, 100 and 150 mm. Guards with length of
10 mm and the inner diameters 2, 3 and 4 mm are also available.
Complete list of availible column dimensions
If you have any questions regarding the above, don´t hesitate to
contact ChromTech!
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